8X7C

Crystal structure of ZmHSL1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.06
Spacegroup name	C 1 2 1
Unit cell lengths	88.062, 54.368, 174.744
Unit cell angles	90.00, 104.34, 90.00

Refinement procedure

Resolution	26.840 - 1.597
R-factor	0.175
Rwork	0.173
R-free	0.20370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.620
High resolution limit [Å]	1.597	1.600
Rmerge	0.042	0.374
Number of reflections	106167	3167
<I/σ(I)>	16.33	2.68
Completeness [%]	99.4
Redundancy	3.5
CC(1/2)	0.999	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	MES, PEG 4000