8X70
The Crystal Structure of IFI16 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.400, 87.418, 111.214 |
| Unit cell angles | 90.00, 99.27, 90.00 |
Refinement procedure
| Resolution | 46.523 - 1.700 |
| Rwork | 0.201 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.523 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | 0.798 |
| Number of reflections | 83684 | 4406 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15M K bromide, 30% w/v PEG 2000 MME |
