8X6P
Isomerase Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-01 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.850, 36.124, 54.499 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 22.080 - 1.050 |
| R-factor | 0.2054 |
| Rwork | 0.205 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.900 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 101614 | 6791 |
| <I/σ(I)> | 16.16 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.075 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | NaCl, Ammonium Sulfate, TRIS |
