8X5V

BlCas9-sgRNA-target DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	144.750, 99.210, 119.300
Unit cell angles	90.00, 97.09, 90.00

Refinement procedure

Resolution	118.389 - 2.000
Rwork	0.198
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.615
Data reduction software	DIALS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	118.389	50.000	2.120
High resolution limit [Å]	2.000	6.000	2.000
Rmerge	0.250	0.076	4.111
Rmeas		0.078	4.186
Number of reflections	112881	4301	18007
<I/σ(I)>	19.01
Completeness [%]	99.9
Redundancy	28
CC(1/2)		0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM sodium-acetate pH 4.5, 15%-20% PEG 500 MME, 200 mM ammonium sulfate, 10 mM strontium chloride