8X5U

Crystal structure of Thermus thermophilus peptidyl-tRNA hydrolase C-terminal 16 amino acid deletion mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-11-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.980
Spacegroup name	P 61 2 2
Unit cell lengths	81.948, 81.948, 123.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.970 - 2.100
R-factor	0.20361
Rwork	0.201
R-free	0.24792
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.970	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.062	0.277
Number of reflections	15036	716
<I/σ(I)>	40.2	7.5
Completeness [%]	100.0	100
Redundancy	18.8	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M (NH4)2SO4, 0.1 M Tris-HCl pH7.5, 30% (v/v) PEG600, and 10% (v/v) glycerol