8X5M
The Crystal Structure of JNK1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.170, 128.405, 82.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.786 - 2.000 |
| Rwork | 0.193 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.786 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | 0.814 |
| Number of reflections | 28200 | 2057 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Nacacodylate pH6.0, 15% PEG 4000 |
