8X34
ProteinMY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2023-10-11 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.644, 75.825, 218.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.150 - 2.800 |
| R-factor | 0.2923 |
| Rwork | 0.289 |
| R-free | 0.32850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.150 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.904 | 0.992 |
| Number of reflections | 24347 | 3125 |
| <I/σ(I)> | 2.07 | |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 49.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 4.0 M Ammonium acetate, 0.1 M Tris pH 8.5 |
