8X34
Human Kelch like-ECH Associated Protein 1 (Keap1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2023-10-11 |
Detector | RIGAKU HyPix-3000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.644, 75.825, 218.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.150 - 3.000 |
R-factor | 0.2754 |
Rwork | 0.275 |
R-free | 0.32910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.363 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.150 | 3.020 |
High resolution limit [Å] | 3.000 | 3.010 |
Rmerge | 0.010 | 0.992 |
Number of reflections | 25899 | 3125 |
<I/σ(I)> | 2.07 | |
Completeness [%] | 99.8 | 97.9 |
Redundancy | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 4.0 M Ammonium acetate, 0.1 M Tris pH 8.5 |