8X2O
RIPK2 in complex with K252
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.080, 102.770, 125.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.900 - 2.260 |
| R-factor | 0.2152 |
| Rwork | 0.213 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-3000 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 2.341 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.025 | 0.362 |
| Number of reflections | 37633 | 3690 |
| <I/σ(I)> | 16.97 | 2.19 |
| Completeness [%] | 99.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.729 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Mes pH7,12% PEG 400, 250mM CaCl2 |
