8X28
Crystal structure of H5 hemagglutinin from human-infecting H5N8 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.932, 267.380, 131.188 |
Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
Resolution | 32.040 - 3.190 |
R-factor | 0.2423 |
Rwork | 0.242 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.691 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 267.380 | 3.270 |
High resolution limit [Å] | 3.190 | 3.190 |
Rmerge | 0.349 | 1.999 |
Number of reflections | 82156 | 6061 |
<I/σ(I)> | 5.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 3 % dextran sulfate sodium salt, 0.1 M bicine pH 7.7, 18 % w/v PEG20000 |