8X1H

Crystal structure of N-terminal domain of Nucleocapsid protein of SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2022-03-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	58.945, 92.344, 96.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.240 - 2.000
R-factor	0.2396
Rwork	0.237
R-free	0.27780
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.220	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.043	0.478
Rmeas	0.048	0.534
Rpim	0.021	0.234
Total number of observations	182143
Number of reflections	36178	2653
<I/σ(I)>	22.9	3.5
Completeness [%]	100.0	100
Redundancy	5	5.1
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	27 % PEG3350, Bicine buffer pH 9.0