8X1H
Crystal structure of N-terminal domain of Nucleocapsid protein of SARS-CoV-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-03-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.945, 92.344, 96.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.240 - 2.000 |
R-factor | 0.2396 |
Rwork | 0.237 |
R-free | 0.27780 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.549 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.220 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.043 | 0.478 |
Rmeas | 0.048 | 0.534 |
Rpim | 0.021 | 0.234 |
Total number of observations | 182143 | |
Number of reflections | 36178 | 2653 |
<I/σ(I)> | 22.9 | 3.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5 | 5.1 |
CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 27 % PEG3350, Bicine buffer pH 9.0 |