8X1A
Crystal structure of periplasmic G6P binding protein VcA0625
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 294.15 |
Detector technology | IMAGE PLATE |
Collection date | 2018-05-24 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.288, 63.312, 75.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.470 - 1.604 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.19630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.410 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.600 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.344 | |
Number of reflections | 36010 | 1545 |
<I/σ(I)> | 19.8 | |
Completeness [%] | 98.7 | |
Redundancy | 4.6 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 294.15 | PEG 400, phosphate-citrate |