8X0N

The molecular mechanism of hnRNPA1 recognize TERRA RNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	101.298, 101.298, 55.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.824 - 2.800
R-factor	0.2385
Rwork	0.234
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.458
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.158	0.523
Number of reflections	7512	350
<I/σ(I)>	9
Completeness [%]	99.9
Redundancy	5.7
CC(1/2)	0.976	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	100mM HEPES, pH7.0, 10% PEG 4000, 10% 2-Propanol