8X0C
Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate and VU0424465, conformer 1
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Number of Particle | 4481289 |
| Software | Topaz,Coot,cryoSPARC,PHENIX,REFMAC,EPU,CTFFIND,RELION,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
