8WZN

ParkinK211N in complex with phospho NEDD8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	58.249, 74.603, 80.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.860 - 1.800
Rwork	0.179
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ikv
RMSD bond length	0.006
RMSD bond angle	1.479
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.860	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	32755	3236
<I/σ(I)>	10.25
Completeness [%]	99.2
Redundancy	4.5
CC(1/2)	0.996	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M, MMT, 7.0, 25% w/v, PEG 1500