8WZG
SFX structure of an Mn-carbonyl complex immobilized in hen egg white lysozyme microcrystals, 10 ns after photoexcitation at RT.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2020-11-24 |
| Detector | MPCCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.000, 81.000, 37.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.490 - 1.600 |
| R-factor | 0.1865 |
| Rwork | 0.186 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.490 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 17136 | 841 |
| <I/σ(I)> | 8.04 | |
| Completeness [%] | 100.0 | |
| Redundancy | 471.62 | |
| CC(1/2) | 0.980 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 3 | 293 | 0.1M Acetate buffer (pH3.0), 28% NaCl, 8% PEG 6000 |
