8WZF

Dark state SFX structure of hen egg white lysozyme microcrystals immobilized with a Mn carbonyl complex at RT.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	298
Detector technology	CCD
Collection date	2020-11-24
Detector	MPCCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	81.000, 81.000, 37.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.490 - 1.600
R-factor	0.195
Rwork	0.193
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.490	1.620
High resolution limit [Å]	1.600	1.600
Number of reflections	17136	841
<I/σ(I)>	6.82
Completeness [%]	100.0
Redundancy	448.21
CC(1/2)	0.965	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	293	0.1M Acetate buffer (pH3.0), 28% NaCl