8WZD

The Crystal Structure of PKCi from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1806
Spacegroup name	P 21 21 21
Unit cell lengths	45.760, 75.047, 80.266
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.165 - 2.050
Rwork	0.171
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.760	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.149	0.942
Number of reflections	17997	1368
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Na2tartrate, 0.1M Hepes pH7.0, 20% SOKALAN PA25 CL