8WZC

NYN domain of human KHNYN complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97495
Spacegroup name	P 41 3 2
Unit cell lengths	111.197, 111.197, 111.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.729 - 1.925
Rwork	0.180
R-free	0.20940
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Number of reflections	18429	1243
<I/σ(I)>	53.2875
Completeness [%]	99.9
Redundancy	104
CC(1/2)	0.999	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-HCl, PEG4000, calcium chloride, 1-Propanol