8WXS
Crystal structure of the LOTUS domain of Drosophila Tejas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97928 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 36.645, 36.645, 235.485 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.920 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.186 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.130 |
| Number of reflections | 11648 | 1022 |
| <I/σ(I)> | 54.89 | |
| Completeness [%] | 98.5 | |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2 M NH4Ac, 0.1 M NaAc, pH 4.6, 30% PEG 4000 |
