8WXO
Crystal structure of substrate-binding protein from Rhodothermus marinus (Dose III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.341, 64.122, 69.757 |
Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
Resolution | 39.080 - 1.680 |
R-factor | 0.2201 |
Rwork | 0.218 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.454 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.090 | 1.740 |
High resolution limit [Å] | 1.680 | 1.680 |
Number of reflections | 61756 | 3159 |
<I/σ(I)> | 6.4 | |
Completeness [%] | 99.8 | |
Redundancy | 6 | |
CC(1/2) | 0.996 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, MgCl2, polyethylene glycol 3350 |