8WXN
Crystal structure of substrate-binding protein from Rhodothermus marinus (Dose II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 63.552, 64.164, 69.863 |
Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
Resolution | 36.550 - 1.580 |
R-factor | 0.2235 |
Rwork | 0.222 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.970 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.560 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 74744 | 3822 |
<I/σ(I)> | 7 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 6 | 6 |
CC(1/2) | 0.997 | 0.656 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, MgCl2, polyethylene glycol 3350 |