8WXM
Crystal structure of substrate-binding protein from Rhodothermus marinus (Dose I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.577, 63.925, 69.698 |
| Unit cell angles | 90.00, 96.04, 90.00 |
Refinement procedure
| Resolution | 39.070 - 1.500 |
| R-factor | 0.2207 |
| Rwork | 0.220 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.090 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 85769 | 4052 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 98.8 | 91.6 |
| Redundancy | 5.8 | 4.5 |
| CC(1/2) | 0.997 | 0.587 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, MgCl2, polyethylene glycol 3350 |
