8WVB
Crystal structure of Lsd18 mutant S195M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.041, 48.671, 136.049 |
| Unit cell angles | 90.00, 91.34, 90.00 |
Refinement procedure
| Resolution | 30.114 - 2.500 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8up4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.549 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.114 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.370 | 0.994 |
| Rmeas | 0.402 | 1.077 |
| Rpim | 0.153 | 0.408 |
| Number of reflections | 28322 | 2798 |
| <I/σ(I)> | 3.9 | 2.6 |
| Completeness [%] | 99.0 | 98.52 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.918 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350 |
