8WV8
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 63 |
Unit cell lengths | 95.191, 95.191, 184.086 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.220 - 2.930 |
R-factor | 0.2783 |
Rwork | 0.276 |
R-free | 0.33510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 1.234 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.220 | 3.030 |
High resolution limit [Å] | 2.930 | 2.930 |
Rmerge | 0.117 | |
Number of reflections | 20257 | 1985 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.7 | |
Redundancy | 10.3 | |
CC(1/2) | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 313 | sodium/pottasium tartrae, sodium acetate, KCl |