8WUG
The Crystal Structure of JMJD2D from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.340, 71.340, 150.873 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.887 - 1.700 |
Rwork | 0.161 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.131 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.890 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.029 | 0.162 |
Number of reflections | 40301 | 2235 |
<I/σ(I)> | 60.6 | 13.4 |
Completeness [%] | 92.0 | |
Redundancy | 15.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES, NaOH pH 7.5, 2% PEG 400, 2M Ammonium Sulfate |