8WTB
Crystal structure of McsA/McsB complex truncated by chymotrypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.869, 75.590, 79.190 |
| Unit cell angles | 90.00, 99.78, 90.00 |
Refinement procedure
| Resolution | 88.630 - 2.900 |
| R-factor | 0.2079 |
| Rwork | 0.205 |
| R-free | 0.25870 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.164 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19rc5_4047: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.630 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.157 | 1.124 |
| Number of reflections | 22473 | 3682 |
| <I/σ(I)> | 8.1 | 1.4 |
| Completeness [%] | 96.2 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | 8.25% PEG 400, 6.6% PEG 3350, 0.1 M MgCl2, 0.1 M Tris/HCl pH 8.5 |
