8WT4
Crystal structure of DL-endopeptidase CwlO complexed with IseA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.200, 58.840, 69.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.440 - 1.620 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.685 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.200 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.086 | 0.759 |
| Rmeas | 0.096 | 0.826 |
| Rpim | 0.046 | 0.410 |
| Number of reflections | 47169 | 2375 |
| <I/σ(I)> | 10 | 1.7 |
| Completeness [%] | 96.9 | 99.5 |
| Redundancy | 4.22 | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 30% (v/v) isopropanol, 15% (v/v) PEG 3350, 0.2 M ammonium citrate/citric acid pH 7.5 |
