8WT0

The toxin-antitoxin complex Fic-1-AntF is a deAMPylase that regulates the activity of DNA gyrase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 63 2 2
Unit cell lengths	107.026, 107.026, 113.529
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.410 - 2.500
Rwork	0.212
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.410	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.104	1.187
Rmeas	0.107	1.221
Rpim	0.024	0.281
Number of reflections	13820	1324
<I/σ(I)>	23.02	3.07
Completeness [%]	99.7	99.4
Redundancy	19.1
CC(1/2)	1.000	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.5 M Li2SO4, 0.1M MES pH 5.6, 0.01 M MgCl2