8WSU
Crystal structure of SFTSV Gc and antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-14 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 1.03879 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 229.515, 72.053, 112.360 |
| Unit cell angles | 90.00, 111.47, 90.00 |
Refinement procedure
| Resolution | 36.030 - 3.300 |
| R-factor | 0.2101 |
| Rwork | 0.207 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.079 | 0.444 |
| Number of reflections | 25148 | 2106 |
| <I/σ(I)> | 8 | 1.2 |
| Completeness [%] | 95.3 | 80.5 |
| Redundancy | 7.9 | 5.6 |
| CC(1/2) | 0.984 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 291 | 0.1M Tris, pH7.8, 5% w/v gamma-PGA (Na+ form, LM), 15% w/v PEG 4000 |
