8WQJ

Crystal Structure of Transaminase from Shimia marina

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	64.710, 73.440, 184.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.610 - 2.140
R-factor	0.1778
Rwork	0.176
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	2.216
High resolution limit [Å]	2.140	2.140
Number of reflections	49439	4867
<I/σ(I)>	10.95
Completeness [%]	99.8
Redundancy	9
CC(1/2)	0.994	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M BIS-TRIS pH 6.5 25% w/v Polyethylene glycol 3350