8WPT

Truncated mutant (1-171) of ferritin from Ureaplasma diversum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	F 4 3 2
Unit cell lengths	181.600, 181.600, 181.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.103 - 2.360
R-factor	0.1876
Rwork	0.182
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.420	2.420
High resolution limit [Å]	2.360	2.360
Rmerge	0.161	2.007
Rmeas	0.163	2.033
Rpim	0.026	0.324
Total number of observations		30778
Number of reflections	19904	793
<I/σ(I)>	24.4	2.8
Completeness [%]	99.9
Redundancy	37.6	38.8
CC(1/2)	1.000	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Bicine, MgCl2