8WOH

human dsDNA sensor CCDC25 complexed with 18bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	71.660, 77.964, 99.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.200 - 3.200
R-factor	0.233
Rwork	0.230
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2
RMSD bond length	0.011
RMSD bond angle	1.286
Data reduction software	autoPROC
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.072	1.274
Rmeas	0.078	1.370
Rpim	0.029	0.498
Number of reflections	9651	1376
<I/σ(I)>	15.4	1.6
Completeness [%]	99.8	100
Redundancy	7.1	7.4
CC(1/2)	0.999	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH 5.0, 10% MPD pH 6.0