8WO8

Crystal Structure of an RNA-binding protein, FAU-1, from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-17
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 3 2 1
Unit cell lengths	119.573, 119.573, 93.472
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.010 - 2.780
R-factor	0.20985
Rwork	0.206
R-free	0.26797
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q8U4Q7-F1-model_v4
RMSD bond length	0.009
RMSD bond angle	1.733
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.780	2.670
High resolution limit [Å]	2.580	2.670	2.580
Rmerge	0.121	0.937
Number of reflections	22452	1704	937
<I/σ(I)>	12	1.17	0.48
Completeness [%]	90.2	93.5	38.1
Redundancy	9.4	5.7	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	40%(v/v) PEG-300, 0.1 mM HEPES (pH 7.5), 0.2 M NaCl