8WMS

Crystal structure of human DPPA3 in complex with human UHRF1 PHD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	I 41 2 2
Unit cell lengths	77.802, 77.802, 140.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.330 - 2.400
R-factor	0.2346
Rwork	0.233
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.803
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.330	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.064	0.488
Number of reflections	8775	903
<I/σ(I)>	12.5	2.5
Completeness [%]	99.7
Redundancy	5.4
CC(1/2)	0.990	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl (pH 7.0), 200 mM tri-potassium phosphate and 20% (w/v) PEG3,350