8WM2

Crystal structure of AbmM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	70.790, 81.026, 131.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 2.500
R-factor	0.2325
Rwork	0.229
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.240	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.084	0.846
Number of reflections	26915	2974
<I/σ(I)>	14.2	2.1
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.998	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium chloride, 0.1 M HEPES (pH 7.5), 1.6 M ammonium sulfate