8WLG

Crystal structure of Cypovirus Polyhedra mutant fused with c-Myc fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	I 2 3
Unit cell lengths	105.140, 105.140, 105.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.170 - 2.550
R-factor	0.2214
Rwork	0.216
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.550	2.550
Number of reflections	6468	1003
<I/σ(I)>	11.44
Completeness [%]	100.0
Redundancy	78.8
CC(1/2)	0.990	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization