8WL9
X-ray structure of Enterobacter cloacae allose-binding protein in complex with D-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.850, 110.680, 37.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.290 - 1.930 |
| R-factor | 0.24109 |
| Rwork | 0.239 |
| R-free | 0.28978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rpj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.152 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.290 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 20335 | 1440 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.995 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 10 mM D-ribose, 100 mM sodium citrate tribasic dihydrate, 22% (w/v) polyethylene glycol 1,000 |
