8WJB

Structural insights into the Langya virus attachment glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	C 1 2 1
Unit cell lengths	196.767, 48.564, 116.924
Unit cell angles	90.00, 122.67, 90.00

Refinement procedure

Resolution	46.310 - 2.750
R-factor	0.2193
Rwork	0.216
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.211
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.750	2.750
Number of reflections	24224	2116
<I/σ(I)>	5.15
Completeness [%]	99.6
Redundancy	5.7
CC(1/2)	0.926	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Magnesium chloride hexahydrate, Sodium HEPES 7.5, PEG 400