8WIP
Crystal Structure of Pseudomonas aeruginosa SuhB in its apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-08-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.784, 89.358, 98.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.600 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.710 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 18511 | 2666 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.997 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Sodium acetate trihydrate, PEG 3350, pH 5.0 |
