8WIM
Crystal structure of Jingmen tick virus RNA-dependent RNA polymerase (D307 construct)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.522, 89.251, 122.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.110 - 1.840 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.20920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house model determined by SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.918 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.12) |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.910 |
High resolution limit [Å] | 1.840 | 3.960 | 1.840 |
Rmerge | 0.077 | 0.036 | 0.583 |
Rmeas | 0.083 | 0.039 | 0.671 |
Rpim | 0.031 | 0.014 | 0.320 |
Total number of observations | 395284 | ||
Number of reflections | 58904 | 6204 | 5474 |
<I/σ(I)> | 9.6 | ||
Completeness [%] | 99.1 | 98.9 | 93.5 |
Redundancy | 6.7 | 7.1 | 3.6 |
CC(1/2) | 0.999 | 0.999 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | potassium sodium tartrate tetrahyrate |