8WIL
Crystal structure of Jingmen tick virus RNA-dependent RNA polymerase (D55 construct)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 289 |
Detector technology | PIXEL |
Collection date | 2019-12-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.519, 89.185, 121.768 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.760 - 2.100 |
R-factor | 0.1734 |
Rwork | 0.172 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house model determined by SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.000 | 60.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.096 | 0.046 | 0.508 |
Rmeas | 0.098 | 0.047 | 0.522 |
Rpim | 0.022 | 0.011 | 0.119 |
Total number of observations | 759365 | ||
Number of reflections | 40074 | 4254 | 3935 |
<I/σ(I)> | 6.4 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 18.9 | 17.9 | 19.1 |
CC(1/2) | 1.000 | 0.999 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | magnesium chloride, HEPES pH7.5, PEG3350 |