8WIK

Crystal structure of human FSP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-01-15
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	56.875, 56.875, 505.105
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.260 - 2.000
R-factor	0.227
Rwork	0.225
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.118	0.072	1.621
Rmeas	0.125	0.076	1.718
Rpim	0.040	0.026	0.559
Total number of observations	339711
Number of reflections	34829	3969	3353
<I/σ(I)>	4.6
Completeness [%]	100.0	99.9	100
Redundancy	9.8	8.8	9.2
CC(1/2)	0.969	0.989	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	HEPES , MgCl2, PEG3350