8WHJ
Crystal structure of CLASP2 TOG4 fused with LL5beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 |
Unit cell lengths | 51.132, 51.132, 97.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.566 - 1.400 |
R-factor | 0.1311 |
Rwork | 0.129 |
R-free | 0.17350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.172 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
Rmerge | 0.064 | 0.036 | 0.822 |
Rmeas | 0.067 | 0.037 | 0.866 |
Rpim | 0.019 | 0.010 | 0.267 |
Number of reflections | 49279 | 2524 | 2432 |
<I/σ(I)> | 7.2 | ||
Completeness [%] | 99.8 | 99.9 | 99.8 |
Redundancy | 11.7 | 12.2 | 9.7 |
CC(1/2) | 1.000 | 0.999 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Sodium chloride, 0.1 M Bis-Tris pH 5.5, and 25% w/v PEG 3350 |