8WGL
Crystal structure of Rhodothermus marinus substrate-binding protein (Hg soaking)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.645, 45.209, 57.041 |
Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
Resolution | 24.110 - 2.300 |
R-factor | 0.1973 |
Rwork | 0.192 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.663 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.373 |
Number of reflections | 11062 | 570 |
<I/σ(I)> | 21.83 | |
Completeness [%] | 96.4 | 100 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Bis-Tris, pH 6.5, Magnesium chloride and Polyethylene glycol 3,350 |