8WG0
Crystal structure of GH97 glucodextranase from Flavobacterium johnsoniae in complex with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-05 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 307.927, 103.691, 95.671 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.130 - 1.950 |
Rwork | 0.189 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.902 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.090 | 1.471 |
Rmeas | 0.097 | 1.588 |
Rpim | 0.037 | 0.594 |
Number of reflections | 223030 | 10951 |
<I/σ(I)> | 23 | 2.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 13.5 | 13.6 |
CC(1/2) | 1.000 | 0.836 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG20000, 200 mM sodium cacodylate buffer (pH 6.0), 200 mM magnesium acetate, 10 mM glucose |