8WFW

Crystal structure of beta-glucosidase from Thermoanaerobacterium saccharolyticum (Data 4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	65.066, 71.211, 99.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 1.610
R-factor	0.1515
Rwork	0.151
R-free	0.17620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.963
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	60041	2923
<I/σ(I)>	14.63
Completeness [%]	98.8
Redundancy	5.7
CC(1/2)	0.981	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Tris-HCl, PEG 4000, MgCl2