8WFV
Crystal structure of beta-glucosidase from Thermoanaerobacterium saccharolyticum (Data 3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.970, 70.948, 98.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.440 - 1.500 |
R-factor | 0.155 |
Rwork | 0.155 |
R-free | 0.17120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.648 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.440 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 71920 | 3026 |
<I/σ(I)> | 15.41 | |
Completeness [%] | 97.4 | |
Redundancy | 4.1 | |
CC(1/2) | 0.973 | 0.771 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Tris-HCl, PEG 4000, MgCl2 |