8WFE

The Crystal Structure of PPARg from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95374
Spacegroup name	C 1 2 1
Unit cell lengths	91.808, 62.297, 118.415
Unit cell angles	90.00, 101.73, 90.00

Refinement procedure

Resolution	49.030 - 2.200
Rwork	0.217
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.051	0.751
Number of reflections	32713	2669
<I/σ(I)>	16.6
Completeness [%]	97.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MIB pH9.0, 25% PEG 1500