8WFC
N5, N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2023-05-03 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 110.681, 110.681, 69.781 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.050 |
R-factor | 0.2161 |
Rwork | 0.215 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.100 | 0.304 |
Number of reflections | 27821 | 1347 |
<I/σ(I)> | 59.67 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | Sodium Potassium phosphate, MPD |