8WEW

Haloquadratum walsbyi middle rhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	61.460, 62.470, 122.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.810 - 2.500
R-factor	0.28968
Rwork	0.287
R-free	0.33446
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.383
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.2-4158)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.810	2.690
High resolution limit [Å]	2.500	2.500
Number of reflections	13299	2272
<I/σ(I)>	5.64
Completeness [%]	78.8
Redundancy	14.1
CC(1/2)	0.970	0.241

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	200mM Lithium sulfate, 100mM imidazole, 30% PEG 400